Abstract
Culturable psychrophilic prokaryotes were obtained of samples of glacier sediment, seaside mud, glacier melted ice, and Deschampsia antarctica rhizosphere from Collins glacier, Antarctica. The taxonomic classification was done by a culture-dependent molecular approach involving the Amplified Ribosomal DNA Restriction Analysis. Two hundred sixty colonies were successfully isolated and sub-cultivated under laboratory conditions. The analysis showed a bacterial profile dominated by Beta-proteobacteria (35.2%) followed by Gamma-proteobacteria (18.5%), Alpha-proteobacteria (16.6%), Gram-positive with high GC content (13%), Cytophaga-Flavobacterium-Bacteroides (13%) and Gram-positive with low GC content (3.7%). Eleven of the isolates have been reported previously and the others microorganisms remain uncharacterized. The isolated microorganisms here could be a potential source for biotechnological products, such as cold-active enzymes and secondary metabolites.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antarctic Regions
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Bacteroides / genetics*
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Bacteroides / isolation & purification*
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Biological Products / isolation & purification
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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DNA, Ribosomal / analysis
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DNA, Ribosomal / genetics
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Geologic Sediments / microbiology*
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Ice Cover / microbiology*
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Molecular Sequence Data
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Phylogeny*
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Proteobacteria / genetics*
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Proteobacteria / isolation & purification*
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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Biological Products
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/EU636014
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GENBANK/EU636015
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GENBANK/EU636016
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GENBANK/EU636017
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GENBANK/EU636018
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GENBANK/EU636019
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GENBANK/EU636020
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GENBANK/EU636021
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GENBANK/EU636022
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GENBANK/EU636023
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GENBANK/EU636024
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GENBANK/EU636025
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GENBANK/EU636026
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GENBANK/EU636027
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GENBANK/EU636028
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GENBANK/EU636029
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GENBANK/EU636030
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GENBANK/EU636031
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GENBANK/EU636032
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GENBANK/EU636033
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GENBANK/EU636034
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GENBANK/EU636035
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GENBANK/EU636036
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GENBANK/EU636037
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GENBANK/EU636038
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GENBANK/EU636039
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GENBANK/EU636040
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GENBANK/EU636041
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GENBANK/EU636042
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GENBANK/EU636061
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GENBANK/EU636062
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GENBANK/EU636063
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GENBANK/EU636064
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GENBANK/EU636065