Characterization of nuclear microsatellite loci in the Neotropical tree Parkia panurensis (Fabaceae)

Am J Bot. 2010 May;97(5):e34-6. doi: 10.3732/ajb.1000096. Epub 2010 Apr 19.

Abstract

Premise of the study: We present here a set of nine polymorphic nuclear microsatellite loci, identified for the first time within the neotropical legume tree species Parkia panurensis Benth. ex H. C. Hopkins, which is widespread in western and central Amazonia.

Methods and results: To characterize these loci, 33 Parkia panurensis adult trees were analyzed. The number of alleles ranged from eight to 32, with an average of 14.4 alleles per locus. Mean expected heterozygosity ranged from 0.74 to 0.955.

Conclusions: All nine loci could also be verified in six other Parkia species and polymorphic fragments amplified. The new marker set can be used for future studies of genetic diversity and differentiation, as well as estimation of gene flow and parentage analyses in various Parkia species.