This study sought to evaluate the influence of a dentin desensitizer and ozone application on the bond strength to dentin of a composite resin material. The dentin desensitizing agent and ozone treatment were applied on the cervical dentin surfaces of extracted, caries-free, erupted third molars. Dentin surfaces that received no treatment were used as control samples. A dentin bonding agent was applied according to the manufacturer's instructions and an adhesion test was performed according to ISO/TS 11405. Statistical analysis showed no significant influence of the different hypersensitivity treatments on shear bond strength to dentin (ANOVA and Tukey's tests, p > 0.05). Within the limitations of this in vitro study, it appears that the short-term use of dentin hypersensitivity treatments like ozone and dentin desensitizers containing gluteraldehyde do not further affect the shear bond strength to dentin of subsequent composite resin restorations.