Rickettsia, Ehrlichia, Anaplasma, and Bartonella in ticks and fleas from dogs and cats in Bangkok

Vector Borne Zoonotic Dis. 2011 Oct;11(10):1335-41. doi: 10.1089/vbz.2010.0174. Epub 2011 May 25.

Abstract

Flea and tick specimens (5-10 fleas or ticks) on dogs and cats from various sites in Bangkok were tested by polymerase chain reaction and DNA sequencing to detect DNA of bacteria Rickettsia (gltA and 17 kDa genes), Anaplasmataceae (16S rRNA gene), and Bartonella (pap31 and its genes). We confirmed that Rickettsia sp. related to Rickettsia felis was detected in 66 of 98 (67.4%) flea specimens from dogs, whereas 8 Bartonella henselae and 2 Bartonella clarridgeiae were detected in 10 of 54 (18.5%) flea specimens from cats. Further, this work provides the first evidence of 10 Ehrlichia canis (3.3%), 7 Anaplasma platys (2.3%), and 2 Wolbachia spp. (0.66%) in 304 Rhipicephalus sanguineus tick specimens in Thailand.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaplasma / genetics
  • Anaplasma / isolation & purification*
  • Animals
  • Bartonella / genetics
  • Bartonella / isolation & purification*
  • Cats
  • Ctenocephalides / microbiology*
  • DNA Primers
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Dogs
  • Ehrlichia / genetics
  • Ehrlichia / isolation & purification*
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Rhipicephalus / microbiology*
  • Rickettsia / genetics
  • Rickettsia / isolation & purification*
  • Sequence Analysis, DNA
  • Species Specificity
  • Thailand

Substances

  • DNA Primers
  • DNA, Bacterial

Associated data

  • GENBANK/JF511463
  • GENBANK/JF511464
  • GENBANK/JF511465
  • GENBANK/JF511466