Cloning and expression of one chloroplastic ascorbate peroxidase gene from Nelumbo nucifera

Biochem Genet. 2011 Oct;49(9-10):656-64. doi: 10.1007/s10528-011-9440-x. Epub 2011 May 24.

Abstract

A novel ascorbate peroxidase (APX) cDNA was obtained from Nelumbo nucifera (Elian). The phylogenetic analysis indicated that N. nucifera APX grouped together with chloroplastic APX of high plants. The recombinant protein expressed by PET-30a vector showed APX activity (0.04 mM ascorbate min(-1) mg(-1) protein). The APX mRNA was expressed in young leaves, roots, terminal buds, and leafstalks. Synergistic expression of N. nucifera APX and MnSOD mRNA was indicated in the short-term response to mechanical wounding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Ascorbate Peroxidases / biosynthesis
  • Ascorbate Peroxidases / genetics*
  • Base Sequence
  • Chloroplasts / genetics*
  • Cloning, Molecular
  • Gene Expression
  • Gene Expression Regulation, Plant
  • Molecular Sequence Data
  • Nelumbo / enzymology
  • Nelumbo / genetics*
  • Nelumbo / physiology
  • Phylogeny
  • Plant Leaves / genetics
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics*
  • Stress, Physiological
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism

Substances

  • Plant Proteins
  • Recombinant Proteins
  • Ascorbate Peroxidases
  • Superoxide Dismutase