A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

Chem Commun (Camb). 2011 Jul 14;47(26):7452-4. doi: 10.1039/c1cc10442j. Epub 2011 May 20.

Abstract

A rapid and reverse transcription-free real-time PCR microRNA assay was developed based on effects of base stacking. This microRNA assay has been shown to be highly specific to homogenous miRNAs, and the procedure can be completed within 30 min starting from total RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pair Mismatch
  • Base Sequence
  • Cell Line, Tumor
  • Humans
  • MicroRNAs / analysis*
  • MicroRNAs / chemistry*
  • MicroRNAs / genetics
  • Nucleic Acid Conformation*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Time Factors

Substances

  • MicroRNAs