Abstract
The high reactivity of thiyl radicals (RS(•)), which results in half-lives on the order of microseconds, hinders their analysis in biological systems. This Feature reviews the contemporary approaches to assessment of RS(•) using EPR spin trapping, mass spectrometric, immunological, and HPLC protocols.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
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Review
MeSH terms
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Chromatography, High Pressure Liquid
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Electron Spin Resonance Spectroscopy
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Electron Transport
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Fluorescent Dyes / chemistry
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Free Radicals / analysis*
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Glutathione / chemistry
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Humans
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Mass Spectrometry
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Spin Trapping
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Sulfhydryl Compounds / chemistry*
Substances
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Fluorescent Dyes
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Free Radicals
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Sulfhydryl Compounds
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Glutathione