The title compound, C(19)H(28)O(4), was prepared from DHEA (dehydro-epiandrosterone) by its biotransformation using whole cells of the filamentous fungus Beauveria bassiana. The asymmetric unit contains two mol-ecules. The lactone ring is trans-positioned to the neighboring six-membered ring. In the crystal structure, O-H⋯O hydrogen bonds form layers, which are linked to each other by O-H⋯O and C-H⋯O hydrogen bonds.