Efficient molecular sexing in dioecious Silene latifolia and S. dioica and paternity analysis in F(1) hybrids

R Hobza; A Widmer

doi:10.1111/j.1755-0998.2008.02344.x

Efficient molecular sexing in dioecious Silene latifolia and S. dioica and paternity analysis in F(1) hybrids

Mol Ecol Resour. 2008 Nov;8(6):1274-6. doi: 10.1111/j.1755-0998.2008.02344.x.

Authors

R Hobza¹, A Widmer

Affiliation

¹ Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-612 65 Brno, Czech Republic, Plant Ecological Genetics, Institute of Integrative Biology, ETH Zurich, Universitaetstr. 16, 8092 Zurich, Switzerland.

PMID: 21586019
DOI: 10.1111/j.1755-0998.2008.02344.x

Abstract

Two polymerase chain reaction-based assays have been developed that work in combination with an efficient DNA extraction protocol to rapidly and reliably determine sex in the dioecious plant species Silene latifolia and S. dioica. In addition, one of the assays allows assessing paternity in the F(1) generation of intra- and interspecific matings involving the two species.