Microsatellite DNA markers for the Chinese wood frog (Rana chensinensis) and tests for their cross-utility in 15 ranid frog species

Aibin Zhan; Jinzhong Fu

doi:10.1111/j.1755-0998.2008.02192.x

Microsatellite DNA markers for the Chinese wood frog (Rana chensinensis) and tests for their cross-utility in 15 ranid frog species

Mol Ecol Resour. 2008 Sep;8(5):1126-9. doi: 10.1111/j.1755-0998.2008.02192.x.

Authors

Aibin Zhan¹, Jinzhong Fu

Affiliation

¹ Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

PMID: 21585991
DOI: 10.1111/j.1755-0998.2008.02192.x

Abstract

We developed 22 microsatellite markers for the Chinese wood frog (Rana chensinensis) to study the impact of landscape features on its population structure. Thirty-four individuals from one breeding site were examined and 14 loci were polymorphic. The number of alleles, expected heterozygosity and observed heterozygosity varied from two to 14, from 0.0833 to 0.9118, and from 0.1376 to 0.8667, respectively. Cross-species amplification was tested for 15 ranid frog species. The Plateau brown frog, Rana kukunoris (n = 23), was successfully amplified at 18 loci, and 15 were polymorphic with number of alleles varying from two to 18. Ten other species were also amplified at a limited number of loci.