Development of microsatellite markers for the grapevine fungal pathogen Phaeomoniella chlamydospora

Gabrielle Smetham; Peter Ades; Rebecca Ford

doi:10.1111/j.1755-0998.2008.02152.x

Development of microsatellite markers for the grapevine fungal pathogen Phaeomoniella chlamydospora

Mol Ecol Resour. 2008 Sep;8(5):1052-4. doi: 10.1111/j.1755-0998.2008.02152.x. Epub 2008 Jun 28.

Authors

Gabrielle Smetham¹, Peter Ades, Rebecca Ford

Affiliation

¹ BioMarka, School of Agriculture and Food Systems, The University of Melbourne, Melbourne, Victoria 3010, Australia, School of Forest and Ecosystem Science, The University of Melbourne, Melbourne, Victoria 3010, Australia.

PMID: 21585969
DOI: 10.1111/j.1755-0998.2008.02152.x

Abstract

Twenty polymorphic microsatellite markers from microsatellite-enriched genomic DNA of the grapevine fungal pathogen, Phaeomoniella chlamydospora, were developed and characterized. The markers were used to genotype isolates from Australia and from Europe/Eurasia. The number of alleles per locus ranged from two to 11. Gene diversity per locus ranged from 0.08 to 0.63 among Australian isolates, and from 0.2 to 0.77 among isolates from Europe/Eurasia, demonstrating the suitability of these markers for population genetic studies of P. chlamydospora. Eighteen of the 20 markers also amplified a product in the closely related Phaeoacremonium aleophilum.