Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattus L.)

T Dailianis; M Limborg; R Hanel; D Bekkevold; J Lagnel; A Magoulas; C S Tsigenopoulos

doi:10.1111/j.1755-0998.2008.02091.x

Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattus L.)

Mol Ecol Resour. 2008 Jul;8(4):861-3. doi: 10.1111/j.1755-0998.2008.02091.x.

Authors

T Dailianis¹, M Limborg, R Hanel, D Bekkevold, J Lagnel, A Magoulas, C S Tsigenopoulos

Affiliation

¹ Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research Crete, Heraklion, Greece.

PMID: 21585913
DOI: 10.1111/j.1755-0998.2008.02091.x

Abstract

Nine polymorphic microsatellites were isolated from sprat (Sprattus sprattus) using a microsatellite enrichment protocol and selective hybridization with a biotinylated (AC)(12) probe. The loci showed different variation patterns in a Baltic Sea population (44 individuals) with mean number of alleles at 12.7 and mean observed heterozygosity at 0.78. These microsatellite loci are expected to be used for taxonomic considerations in sprat, stock differentiation and population genetic analysis.