PERMANENT GENETIC RESOURCES: Isolation and characterization of polymorphic microsatellite loci in common evening primrose (Oenothera biennis)

E L Larson; S M Bogdanowicz; A A Agrawal; M T J Johnson; R G Harrison

doi:10.1111/j.1471-8286.2007.01983.x

PERMANENT GENETIC RESOURCES: Isolation and characterization of polymorphic microsatellite loci in common evening primrose (Oenothera biennis)

Mol Ecol Resour. 2008 Mar;8(2):434-6. doi: 10.1111/j.1471-8286.2007.01983.x.

Authors

E L Larson¹, S M Bogdanowicz, A A Agrawal, M T J Johnson, R G Harrison

Affiliation

¹ Department of Ecology and Evolutionary Biology, Corson Hall, Cornell University, Ithaca, NY 14853, USA, Department of Biology, Duke University, Durham, NC 27708, USA.

PMID: 21585813
DOI: 10.1111/j.1471-8286.2007.01983.x

Abstract

We developed nine polymorphic microsatellite loci for evening primrose (Oenothera biennis). These loci have two to 18 alleles per locus and observed heterozygosities ranging from 0 to 0.879 in a sample of 34 individuals. In a pattern consistent with the functionally asexual reproductive system of this species, 17/36 pairs of loci revealed significant linkage disequilibrium and three loci showed significant deviations from Hardy-Weinberg equilibrium. The loci will be informative in identifying genotypes in multigenerational field studies to assess changes in genotype frequencies.