Isolation and characterization of microsatellite loci in Psilopeganum sinense Hemsl (Rutaceae), an endangered herb endemic to Yangtze River valley

Feiyan Tang; Qigang Ye; Xiaohong Yao; Hongwen Huang

doi:10.1111/j.1471-8286.2007.01933.x

Isolation and characterization of microsatellite loci in Psilopeganum sinense Hemsl (Rutaceae), an endangered herb endemic to Yangtze River valley

Mol Ecol Resour. 2008 Jan;8(1):227-9. doi: 10.1111/j.1471-8286.2007.01933.x.

Authors

Feiyan Tang¹, Qigang Ye, Xiaohong Yao, Hongwen Huang

Affiliation

¹ Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan, Hubei 430074, China.

PMID: 21585763
DOI: 10.1111/j.1471-8286.2007.01933.x

Abstract

Twenty-nine primer pairs flanking microsatellite repeats were designed from an AC-enriched genomic library of Psilopeganum sinense and tested using 24 individuals derived from a natural population. A total of 11 microsatellite loci were found polymorphic. The number of alleles per locus varied from two to eight, with an average value of 3.7. The ranges of observed and expected heterozygosities were 0.000-0.750 and 0.365-0.800, respectively. These microsatellite loci have been directly applied to the ongoing conservation genetic studies of P. sinense.