Abstract
Objective:
To study the effect of arsenic trioxide (As2O3) and all-trans retinoic acid (ATRA) on human cervical carcinoma HeLa cell line.
Methods:
HeLa cells were treated with As2O3 and ATRA. The cell proliferation was evaluated by MTT assay. The expressions of NDRG-1 protein and mRNA were determined by Western blot and RT-PCR analysis.
Results:
MTT assay showed that As2O3 and ATRA inhibited the growth of human cervical carcinoma HeLa cells in vitro in a dose- and time-dependent manner. Western blot and RT-PCR techniques showed that As2O3 and ATRA down-regulated the expressions of NDRG-1 protein and mRNA (P < 0.05).
Conclusion:
As2O3 and ATRA can significantly inhibit the growth and proliferation of HeLa cells. The reason of these changes may be related with the down-regulation of expression of NDRG-1.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antineoplastic Agents / administration & dosage
-
Antineoplastic Agents / pharmacology*
-
Arsenic Trioxide
-
Arsenicals / administration & dosage
-
Arsenicals / pharmacology*
-
Blotting, Western
-
Cell Cycle Proteins / genetics
-
Cell Cycle Proteins / metabolism*
-
Cell Proliferation / drug effects*
-
Dose-Response Relationship, Drug
-
HeLa Cells
-
Humans
-
Intracellular Signaling Peptides and Proteins / genetics
-
Intracellular Signaling Peptides and Proteins / metabolism*
-
Oxides / administration & dosage
-
Oxides / pharmacology*
-
RNA, Messenger / metabolism
-
Reverse Transcriptase Polymerase Chain Reaction
-
Tretinoin / administration & dosage
-
Tretinoin / pharmacology*
Substances
-
Antineoplastic Agents
-
Arsenicals
-
Cell Cycle Proteins
-
Intracellular Signaling Peptides and Proteins
-
N-myc downstream-regulated gene 1 protein
-
Oxides
-
RNA, Messenger
-
Tretinoin
-
Arsenic Trioxide