Dexamethasone increases neutral sphingomyelinase activity and sphingosine levels in 3T3-L1 fibroblasts

C K Ramachandran; D K Murray; D H Nelson

doi:10.1016/0006-291x(90)92068-b

Dexamethasone increases neutral sphingomyelinase activity and sphingosine levels in 3T3-L1 fibroblasts

Biochem Biophys Res Commun. 1990 Mar 16;167(2):607-13. doi: 10.1016/0006-291x(90)92068-b.

Authors

C K Ramachandran¹, D K Murray, D H Nelson

Affiliation

¹ Department of Medicine, University of Utah School of Medicine, Salt Lake City 84132.

PMID: 2157410
DOI: 10.1016/0006-291x(90)92068-b

Abstract

The activity of neutral sphingomyelinase (EC 3.1.4.12) in a plasma membrane enriched fraction was found to be increased in dexamethasone treated cells. The elevation of sphingomyelinase activity was blocked by cycloheximide indicating that protein synthesis was required for the steroid action. Ceramidase (EC3.5.1.23) activity was unaffected by the dexamethasone treatment. Levels of sphingosine in 3T3-L1 Cells were also increased after treatment with 10(-7) M dexamethasone for 2 and 4 hours.

MeSH terms

Animals
Cell Membrane / enzymology
Cells, Cultured
Cycloheximide / pharmacology
Dexamethasone / pharmacology*
Kinetics
Mice
Phosphoric Diester Hydrolases / metabolism*
Sphingomyelin Phosphodiesterase / metabolism*
Sphingosine / metabolism*

Substances

Dexamethasone
Cycloheximide
Phosphoric Diester Hydrolases
Sphingomyelin Phosphodiesterase
Sphingosine