Molecular epidemiology of the rabies virus in Slovenia 1994-2010

Vet Microbiol. 2011 Aug 26;152(1-2):181-6. doi: 10.1016/j.vetmic.2011.04.019. Epub 2011 Apr 23.

Abstract

A molecular epidemiology study was performed on a selection of 30 rabies-positive brain samples collected between 1994 and 2010 in Slovenia and originating from the red fox (n=19), badger (n=3), cattle (n=3), dog (n=2), cat (n=1), marten (n=1) and horse (n=1). Based on the comparison of 1092 and 672 nucleotide sequences of nucleoprotein (N) and partial glycoprotein (G) gene regions, a low genetic diversity of the circulating strains was detected, but both phylogenetic trees were consistent with the topology where partial nucleoprotein or glycoprotein genes were used. A high sequence identity in the N and G gene to rabies virus isolates from neighbouring countries was found. The Slovenian strains were clearly different from the vaccine strains SAD B19 and SAD Bern, which have been used in Slovenia since 1988.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / genetics
  • Cats / virology
  • Cattle / virology
  • Dogs / virology
  • Foxes / virology
  • Genetic Variation*
  • Glycoproteins / genetics
  • Horses / virology
  • Molecular Epidemiology
  • Mustelidae / virology
  • Nucleoproteins / genetics
  • Phylogeny
  • RNA, Viral / genetics
  • Rabies / epidemiology*
  • Rabies / veterinary
  • Rabies virus / classification
  • Rabies virus / genetics*
  • Rabies virus / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sequence Analysis, RNA
  • Slovenia / epidemiology
  • Viral Envelope Proteins / genetics

Substances

  • Antigens, Viral
  • Glycoproteins
  • Nucleoproteins
  • RNA, Viral
  • Viral Envelope Proteins
  • glycoprotein G, Rabies virus