Microsatellite markers characterized in the barn owl (Tyto alba) and of high utility in other owls (Strigiformes: AVES)

Akos Klein; Gavin J Horsburgh; Clemens Küpper; Agnes Major; Patricia L M Lee; Gyula Hoffmann; Róbert Mátics; Deborah A Dawson

doi:10.1111/j.1755-0998.2009.02715.x

Microsatellite markers characterized in the barn owl (Tyto alba) and of high utility in other owls (Strigiformes: AVES)

Mol Ecol Resour. 2009 Nov;9(6):1512-9. doi: 10.1111/j.1755-0998.2009.02715.x. Epub 2009 May 15.

Authors

Akos Klein¹, Gavin J Horsburgh, Clemens Küpper, Agnes Major, Patricia L M Lee, Gyula Hoffmann, Róbert Mátics, Deborah A Dawson

Affiliation

¹ Department of Animal & Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.

PMID: 21564947
DOI: 10.1111/j.1755-0998.2009.02715.x

Abstract

We have identified 15 polymorphic microsatellite loci for the barn owl (Tyto alba), five from testing published owl loci and 10 from testing non-owl loci, including loci known to be of high utility in passerines and shorebirds. All 15 loci were sequenced in barn owl, and new primer sets were designed for eight loci. The 15 polymorphic loci displayed two to 26 alleles in 56-58 barn owls. When tested in 10 other owl species (n = 1-6 individuals), between four and nine loci were polymorphic per species. These loci are suitable for studies of population structure and parentage in owls.