Development of microsatellite markers for the dwarf bamboo species Sasa cernua and Sasa kurilensis (Poaceae) in northern Japan

Keiko Kitamura; Tomoyuki Saitoh; Ayumi Matsuo; Yoshihisa Suyama

doi:10.1111/j.1755-0998.2009.02675.x

Development of microsatellite markers for the dwarf bamboo species Sasa cernua and Sasa kurilensis (Poaceae) in northern Japan

Mol Ecol Resour. 2009 Nov;9(6):1470-2. doi: 10.1111/j.1755-0998.2009.02675.x. Epub 2009 Apr 12.

Authors

Keiko Kitamura¹, Tomoyuki Saitoh, Ayumi Matsuo, Yoshihisa Suyama

Affiliation

¹ Forest Dynamics and Diversity Group, Hokkaido Research Center, Forestry and Forest Products Research Institute, Sapporo 062-8516, Japan Kiso Experiment Station, Forestry and Forest Products Research Institute, 5473-8 Fukushima, Kiso, Nagano 397-0001, Japan Field Science Center, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi 989-6711, Japan.

PMID: 21564935
DOI: 10.1111/j.1755-0998.2009.02675.x

Abstract

Ten polymorphic microsatellite markers were isolated from the dwarf bamboo species Sasa cernua and Sasa kurilensis. The applicability of these markers was confirmed by genotyping of open-pollinated seeds and leaf samples from natural populations. Genotypes of seeds from each culm shared at least one allele from the maternal parent without contradiction. All 10 loci were polymorphic in S. cernua with 2-15 alleles (average H(E) = 0.532). Eight loci were polymorphic in S. kurilensis with 2-10 alleles (average H(E) = 0.532). These markers will be useful in detailing the extent of clonal and sexual reproduction in these species.