Thirty polymorphic microsatellite loci from the critically endangered kakapo (Strigops habroptilus)

Bruce C Robertson; Nathalie Frauenfelder; Daryl K Eason; Graeme Elliott; Ron Moorhouse

doi:10.1111/j.1755-0998.2008.02506.x

Thirty polymorphic microsatellite loci from the critically endangered kakapo (Strigops habroptilus)

Mol Ecol Resour. 2009 Mar;9(2):664-6. doi: 10.1111/j.1755-0998.2008.02506.x. Epub 2009 Jan 16.

Authors

Bruce C Robertson¹, Nathalie Frauenfelder, Daryl K Eason, Graeme Elliott, Ron Moorhouse

Affiliation

¹ Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand ecogenics GmbH, Wagistrasse 23, 8952 Zurich-Schlieren, Switzerland National Kakapo Team, Department of Conservation, Private Bag 5, Nelson, New Zealand.

PMID: 21564722
DOI: 10.1111/j.1755-0998.2008.02506.x

Abstract

Thirty polymorphic microsatellite loci were developed from the critically endangered kakapo (Strigops habroptilus), using an enriched genomic library. Characterization of loci using 90 kakapo revealed an average of 3.3 alleles per locus (range: 2-5) and an average expected heterozygosity of 0.47 (range: 0.17-0.70). The probability of identity (7.2 × 10(-15) ) and probability of exclusion (0.999999) demonstrate that these loci are a highly informative marker set that can aid the genetic management of the kakapo.