Rapid detection of a cocaine-binding aptamer using biological nanopores on a chip

J Am Chem Soc. 2011 Jun 8;133(22):8474-7. doi: 10.1021/ja2026085. Epub 2011 May 17.

Abstract

This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Aptamers, Nucleotide / metabolism
  • Biosensing Techniques
  • Cocaine / analysis*
  • Cocaine / metabolism
  • Models, Biological
  • Molecular Structure
  • Nanopores*
  • Oligonucleotide Array Sequence Analysis* / methods
  • Time Factors

Substances

  • Aptamers, Nucleotide
  • Cocaine