Abstract
The leaves of Eriobotrya japonica Lindl. have been widely used as a traditional medicine for the treatment of many diseases including gastroenteric disorders, diabetes mellitus, chronic bronchitis and asthma. In the present study, the anti-metastatic action of the EtOAc fraction of the leaves of E. japonica (LEJ) was investigated. LEJ showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of NF-κB translocation to the nucleus in B16F10 cells. In addition, the cell migration and invasion were down-regulated by LEJ. LEJ also significantly suppressed lung metastasis in vivo. Moreover, we isolated the compounds ursolic acid and 2α-hydroxyursolic acid from LEJ and both compounds also significantly suppressed MMP-2 and MMP-9 activities, indicating that they are the active components of LEJ. The present results demonstrate that LEJ may be used as valuable antimetastatic agent for the treatment of cancer metastasis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Antineoplastic Agents, Phytogenic / therapeutic use
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Cell Movement / drug effects
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Cell Nucleus / drug effects
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Cell Nucleus / enzymology
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Cell Proliferation / drug effects
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Eriobotrya / chemistry*
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Humans
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Lung Neoplasms / drug therapy
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Lung Neoplasms / secondary
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Male
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Matrix Metalloproteinase 9
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Matrix Metalloproteinase Inhibitors
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Melanoma, Experimental / drug therapy
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Melanoma, Experimental / secondary
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Mice
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Mice, Inbred C57BL
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Molecular Structure
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NF-kappa B / metabolism
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Neoplasm Invasiveness
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Plant Leaves / chemistry
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Protein Transport
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Xenograft Model Antitumor Assays
Substances
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Antineoplastic Agents, Phytogenic
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Matrix Metalloproteinase Inhibitors
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NF-kappa B
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Plant Extracts
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Matrix Metalloproteinase 9
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Mmp9 protein, mouse