Abstract
Heat shock transcription factor (HSF), an evolutionarily conserved heat-responsive regulator, binds to heat shock elements (HSEs) comprising continuous inverted repeats of the pentamer nGAAn. Here, we analyzed DNA-binding ability, particularly for the discontinuously arranged nGAAn units, of HSFs from various organisms, including Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio. Among these, only C. elegans HSF failed to bind the discontinuous nGAAn units, which suggests that the target genes of HSFs in many, but not all, organisms contain discontinuous HSEs, as well as continuous HSEs. Further analysis of alternatively spliced HSF isoforms showed the importance of the C-terminal region in HSF-HSE interaction.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Base Sequence
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Binding Sites
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Binding, Competitive
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism
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DNA / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Drosophila melanogaster / genetics
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Drosophila melanogaster / metabolism
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Electrophoretic Mobility Shift Assay
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HeLa Cells
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Heat Shock Transcription Factors
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Heat-Shock Response*
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Humans
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Immunoblotting
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Molecular Sequence Data
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Protein Binding
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Repetitive Sequences, Nucleic Acid / genetics
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Response Elements / genetics*
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Sequence Homology, Amino Acid
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Zebrafish / genetics
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Zebrafish / metabolism
Substances
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DNA-Binding Proteins
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Heat Shock Transcription Factors
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Transcription Factors
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DNA