Caged molecular beacons: controlling nucleic acid hybridization with light

Chunming Wang; Zhi Zhu; Yanling Song; Hui Lin; Chaoyong James Yang; Weihong Tan

doi:10.1039/c1cc10481k

Caged molecular beacons: controlling nucleic acid hybridization with light

Chem Commun (Camb). 2011 May 28;47(20):5708-10. doi: 10.1039/c1cc10481k. Epub 2011 Apr 18.

Authors

Chunming Wang¹, Zhi Zhu, Yanling Song, Hui Lin, Chaoyong James Yang, Weihong Tan

Affiliation

¹ State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

PMID: 21499630
DOI: 10.1039/c1cc10481k

Abstract

We have constructed a novel class of light-activatable caged molecular beacons (cMBs) that are caged by locking two stems with a photo-labile biomolecular interaction or covalent bond. With the cMBs, the nucleic acid hybridization process can be easily controlled with light, which offers the possibility for a high spatiotemporal resolution study of intracellular mRNAs.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biotin / chemistry
Fluorescent Dyes / chemistry
Kinetics
Light*
Nucleic Acid Hybridization / methods*
Photolysis
Polyethylene Glycols / chemistry
RNA, Messenger / chemistry

Substances

Fluorescent Dyes
RNA, Messenger
Polyethylene Glycols
Biotin