Radiosynthesis of a new PSMA targeting ligand ([18F]FPy-DUPA-Pep)

Appl Radiat Isot. 2011 Jul;69(7):1014-8. doi: 10.1016/j.apradiso.2011.03.041. Epub 2011 Apr 8.

Abstract

Due to the specificity of expression of PSMA (prostate specific membrane antigen) particularly in prostate cancer cells (e.g. LNCaP), numerous PSMA ligands have been synthesized until now. In the current study, we synthesized DUPA-Pep having 2-[3-(1,3-dicarboxypropyl)ureido]pentanedioic acid (DUPA) linked via 8-aminooctanoic acid to two phenylalanine residues and chose 6-[(18)F]fluoronicotinic acid 2,3,5,6-tetrafluorophenyl ester [(18)F]FPy-TFP as a prosthetic group for coupling. [(18)F]FPy-DUPA-Pep was obtained in a radiochemical yield of 48±0.9% (decay uncorrected) within 50 min with a chemical purity of >98%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Surface / metabolism*
  • Chromatography, High Pressure Liquid
  • Fluorine Radioisotopes / metabolism*
  • Glutamate Carboxypeptidase II / metabolism*
  • Ligands
  • Radiopharmaceuticals / chemical synthesis*
  • Radiopharmaceuticals / metabolism

Substances

  • Antigens, Surface
  • Fluorine Radioisotopes
  • Ligands
  • Radiopharmaceuticals
  • FOLH1 protein, human
  • Glutamate Carboxypeptidase II