The P-selectin gene Pro715 allele and low levels of soluble P-selectin are associated with reduced P2Y12 adenosine diphosphate receptor reactivity in clopidogrel-treated patients

Abstract

Objective: To investigate the interrelation between the P-selectin gene (SELP) Pro715 allele, P2Y12 adenosine diphosphate (ADP) receptor reactivity and levels of soluble P-selectin (sP-selectin) in clopidogrel treated patients.

Methods: The Pro715 allele within SELP was tested by allele specific PCR, sP-selectin was determined by ELISA, and P2Y12 receptor reactivity was analyzed by the VASP assay in 156 patients after angioplasty and stenting.

Results: Carriers of the SELP Pro715 allele had significantly lower levels of sP-selectin and P2Y12 receptor reactivity, and high on-treatment residual P2Y12 receptor reactivity (HRPR) was significantly less frequent compared to non-carriers (both p<0.05). Further, patients within the lowest quartile of sP-selectin had significantly lower reactivity values and also less often HRPR compared to patients with higher sP-selectin (both p<0.01).

Conclusion: The SELP Pro715 allele is linked to low levels of sP-selectin, and both are associated with decreased P2Y12 ADP receptor reactivity in patients on clopidogrel therapy.