LC-MS based quantification of 2'-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

Chem Commun (Camb). 2011 May 14;47(18):5196-8. doi: 10.1039/c1cc11011j. Epub 2011 Mar 29.

Abstract

RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2'-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Monophosphate / analogs & derivatives
  • Base Pairing
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Escherichia coli / chemistry*
  • Escherichia coli / metabolism
  • Guanosine Monophosphate / analogs & derivatives*
  • Guanosine Monophosphate / analysis
  • Guanosine Monophosphate / chemistry
  • Mass Spectrometry
  • Nucleosides / analysis*
  • Nucleosides / chemistry*
  • RNA, Fungal / chemistry*
  • RNA, Fungal / metabolism
  • RNA, Transfer / chemistry*
  • RNA, Transfer / metabolism

Substances

  • Nucleosides
  • RNA, Fungal
  • O-beta-ribosyl(1''-2')-guanosine-5''-phosphate
  • O-beta-ribosyl(1''--2')adenosine-5''-phosphate
  • Adenosine Monophosphate
  • Guanosine Monophosphate
  • RNA, Transfer