A pot experiment was conducted to study the speciation transformation of exogenous dimethyl arsenic (DMA) in soil. The added DMA was mainly transformed into arsenate [As(V)], accompanied with a small amount of monomethyl arsenic (MMA). When the concentration of added DMA was 30 mg x kg(-1), the transformation rate was the highest, being 6.71%, 8.11%, 11.33%, and 19.32% when the cultivation time was 10, 15, 30, and 40 days, respectively. With increasing concentration of added DMA, soil soluble arsenic (AE-As) had an increasing trend, but decreased as the cultivation time increased. Comparing with CK, the addition of DMA increased the concentrations of soil arsenic bounded to aluminum (Al-As), iron (Fe-As), calcium (Ca-As), which was possibly due to the adsorption or fixation of added DMA and its transformation products by the oxides or hydroxides of soil aluminum, iron, and calcium.