Vitamin E supplement and pseudorabies virus (PRV) infection have a reciprocal role in influencing the maternal immune response, a key determinant of the success or failure of pregnancy. However, it remains unknown whether vitamin E supplement provides protection against PRV-induced failure of pregnancy. This study was therefore conducted to investigate the effect of dietary vitamin E level (0, 75, 375, 750 and 1,500 mg/kg) on the reproduction performance, immunity and expression of Toll-like receptors (TLRs) of PRV-challenged mice. The mortality and abortion rate of PRV-challenged mice decreased with the increase in vitamin E consumption. Overall, PBS-injected mice had a higher live embryo number and live litter size than PRV-challenged mice. Both live embryo number and live litter size of PRV-challenged mice increased with increasing vitamin E levels. Vitamin E supplement resulted in decreased concentration of serum IL-2 and IFN-γ, but increased concentration of serum IL-10. The concentration of serum IgG, IgA and IgM increased with increasing vitamin E levels. In the uterine and embryo mRNA abundance of TLR3, TLR7 and TLR9 was higher in PRV-challenged mice than that in PBS-injected mice fed on the same dosage of vitamin E. The mRNA abundance of embryonic TLR3, TLR7 and TLR9 in PRV-challenged mice decreased with increasing vitamin E levels. Collectively, vitamin E supplement may improve reproductive performance of PRV-challenged mice by attenuating PRV-induced negative effects on the cytokine profile, immunoglobulin synthesis and TLR expression.