Objective: To study the effects of neuronal apoptosis under the induction of β-amyloid inflammatory supernatant.
Methods: The neurons were intervened by β-amyloid-induced inflammatory supernatant at the concentration of Aβ1-42 at 125 nmol/L. And the expressions of Bcl-2, caspase-3, PARP and neuronal apoptosis were detected.
Results: The caspase-3 (14.2 ± 1.8), Bcl-2 (10.6 ± 0.8) positive cells of microglia inflammatory supernatant stimulated group were significantly elevated than the control (2.2 ± 0.6, 5.0 ± 0.3, P < 0.01). Nuclear chromatin was uniform yellow-green fluorescent. And there was significant difference of neuronal apoptosis between microglia inflammatory supernatant group and Aβ1-42 directly stimulated group.
Conclusion: Neuronal apoptosis is induced by caspase-3 in β-amyloid inflammatory supernatant. One of the important causes is chronic inflammatory process of activated microglia by Aβ.