Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris

J Biosci Bioeng. 2011 Jun;111(6):654-7. doi: 10.1016/j.jbiosc.2011.02.010. Epub 2011 Mar 9.

Abstract

The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U·l(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Culture Media / chemistry
  • Endo-1,4-beta Xylanases / biosynthesis*
  • Fungal Proteins / biosynthesis
  • Genetic Vectors
  • Industrial Microbiology*
  • Molecular Weight
  • Phanerochaete / enzymology*
  • Phanerochaete / genetics
  • Pichia / metabolism*
  • Promoter Regions, Genetic
  • Protein Sorting Signals
  • Recombinant Proteins / biosynthesis
  • Saccharomyces cerevisiae / genetics

Substances

  • Culture Media
  • Fungal Proteins
  • Protein Sorting Signals
  • Recombinant Proteins
  • Endo-1,4-beta Xylanases