Abstract
The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U·l(-1).
Copyright © 2011. Published by Elsevier B.V.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cloning, Molecular
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Culture Media / chemistry
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Endo-1,4-beta Xylanases / biosynthesis*
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Fungal Proteins / biosynthesis
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Genetic Vectors
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Industrial Microbiology*
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Molecular Weight
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Phanerochaete / enzymology*
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Phanerochaete / genetics
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Pichia / metabolism*
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Promoter Regions, Genetic
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Protein Sorting Signals
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Recombinant Proteins / biosynthesis
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Saccharomyces cerevisiae / genetics
Substances
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Culture Media
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Fungal Proteins
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Protein Sorting Signals
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Recombinant Proteins
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Endo-1,4-beta Xylanases