Abstract
To investigate the expression of the unknown angiotensin II type 2 receptor interacting protein (ATIP) isoforms in the rat we used the known sequences of human and mouse ATIP to design sequencing primers to enable us to sequence rat ATIP3 and ATIP4. Exon 4, which is present in human but not mouse ATIP, was not identified in the coding region of rat ATIP. The expression levels of these genes in a range of rat tissues were examined, and we concluded that there is little similarity in the relative tissue distribution of the various ATIP isoforms in rat and human.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Western
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Cell Line
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Cloning, Molecular
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DNA Primers / genetics
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DNA Primers / metabolism
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Escherichia coli
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Exons
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Humans
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Intracellular Signaling Peptides and Proteins / genetics*
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Intracellular Signaling Peptides and Proteins / metabolism
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Mice
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Molecular Sequence Data
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Organ Specificity
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Polymerase Chain Reaction
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Protein Isoforms / genetics*
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Protein Isoforms / metabolism
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rats
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Receptor, Angiotensin, Type 2 / genetics*
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Receptor, Angiotensin, Type 2 / metabolism
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Recombinant Proteins / genetics*
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Tumor Suppressor Proteins / genetics*
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Tumor Suppressor Proteins / metabolism
Substances
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DNA Primers
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Intracellular Signaling Peptides and Proteins
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Mtus1 protein, rat
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Protein Isoforms
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RNA, Messenger
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Receptor, Angiotensin, Type 2
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Recombinant Proteins
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Tumor Suppressor Proteins