Abstract
An assay based on multiple-locus variable-number tandem-repeat analysis allowed differentiating and studying diversity and persistence of Mycoplasma hyopneumoniae strains in pig herds without prior cultivation. The test had a discriminatory index of >0.99 and was applied reliably to porcine bronchoalveolar lavage fluid and tracheal swabs.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Typing Techniques / methods*
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Bronchoalveolar Lavage Fluid / microbiology
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Cluster Analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Genotype
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Minisatellite Repeats*
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Molecular Sequence Data
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Molecular Typing / methods*
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Mycoplasma hyopneumoniae / classification*
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Mycoplasma hyopneumoniae / genetics
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Mycoplasma hyopneumoniae / isolation & purification*
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Pneumonia of Swine, Mycoplasmal / diagnosis*
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Pneumonia of Swine, Mycoplasmal / microbiology
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Polymorphism, Genetic
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Trachea / microbiology
Associated data
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GENBANK/JF461497
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GENBANK/JF461498
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GENBANK/JF461499
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GENBANK/JF461500
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