Objective: To establish a method for the determination of 5-hydroxy-7-methoxyflavan in Daemrnonorops draco by HPLC.
Method: Dikma Diamonsil C15 column(4. 6 mm x 150 mm, 5 microm) was used. The mobile phase consisted of (60:40). The temperature of column was 30 degrees C. The flow rate was 1:0 mL x min(-1) and the detection wavelength was at 210 nm.
Result: The calibration curve was in good linearity with in the range from 0.01 to 0.10 microg (r = 0.9999). The average recovery was 100.1% (n=6).
Conclusion: The method is simple, rapid, as well as precise and reliable, and can be used for the determination of 5-hydroxy-7-methoxyflavan in D. draco.