Abstract
The effects of RGD peptide conjugation to alginate hydrogel on the adipogenic differentiation of ASCs was investigated. After 3 d of culture, RGD-modified alginate hydrogels significantly stimulated FAK and integrin α1 gene expressions and vinculin expression in ASCs. In addition, RGD-modified alginate hydrogels significantly enhanced the adipogenic differentiation of human ASCs to exhibit higher expression levels of oil red O staining and adipogenic genes compared to those of the control group (unmodified gels). These results suggest potential applications of RGD-modified alginate gels for adipose tissue regeneration.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / cytology
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Adipose Tissue / cytology*
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Adipose Tissue / physiology
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Adult Stem Cells / cytology*
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Adult Stem Cells / drug effects
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Alginates / chemistry*
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Antigens, Differentiation / genetics
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Antigens, Differentiation / metabolism
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / metabolism
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Cell Differentiation / drug effects*
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Cell Survival / drug effects
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Glucuronic Acid / chemistry
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Hexuronic Acids / chemistry
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Humans
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Hydrogels
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Nanoconjugates
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Oligopeptides / chemistry*
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Oligopeptides / pharmacology
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Transcription, Genetic
Substances
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Alginates
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Antigens, Differentiation
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Cell Adhesion Molecules
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Hexuronic Acids
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Hydrogels
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Nanoconjugates
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Oligopeptides
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arginyl-glycyl-aspartic acid
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Glucuronic Acid