The effect of conjugating RGD into 3D alginate hydrogels on adipogenic differentiation of human adipose-derived stromal cells

Macromol Biosci. 2011 May 12;11(5):673-9. doi: 10.1002/mabi.201000479. Epub 2011 Feb 17.

Abstract

The effects of RGD peptide conjugation to alginate hydrogel on the adipogenic differentiation of ASCs was investigated. After 3 d of culture, RGD-modified alginate hydrogels significantly stimulated FAK and integrin α1 gene expressions and vinculin expression in ASCs. In addition, RGD-modified alginate hydrogels significantly enhanced the adipogenic differentiation of human ASCs to exhibit higher expression levels of oil red O staining and adipogenic genes compared to those of the control group (unmodified gels). These results suggest potential applications of RGD-modified alginate gels for adipose tissue regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipose Tissue / cytology*
  • Adipose Tissue / physiology
  • Adult Stem Cells / cytology*
  • Adult Stem Cells / drug effects
  • Alginates / chemistry*
  • Antigens, Differentiation / genetics
  • Antigens, Differentiation / metabolism
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism
  • Cell Differentiation / drug effects*
  • Cell Survival / drug effects
  • Glucuronic Acid / chemistry
  • Hexuronic Acids / chemistry
  • Humans
  • Hydrogels
  • Nanoconjugates
  • Oligopeptides / chemistry*
  • Oligopeptides / pharmacology
  • Transcription, Genetic

Substances

  • Alginates
  • Antigens, Differentiation
  • Cell Adhesion Molecules
  • Hexuronic Acids
  • Hydrogels
  • Nanoconjugates
  • Oligopeptides
  • arginyl-glycyl-aspartic acid
  • Glucuronic Acid