Screening for antimicrobial resistance genes and virulence factors via genome sequencing

Mads Bennedsen; Birgitte Stuer-Lauridsen; Morten Danielsen; Eric Johansen

doi:10.1128/AEM.02493-10

Screening for antimicrobial resistance genes and virulence factors via genome sequencing

Appl Environ Microbiol. 2011 Apr;77(8):2785-7. doi: 10.1128/AEM.02493-10. Epub 2011 Feb 18.

Authors

Mads Bennedsen¹, Birgitte Stuer-Lauridsen, Morten Danielsen, Eric Johansen

Affiliation

¹ Innovation, Chr. Hansen A/S, 10-12 Bøge Allé, DK-2970 Hørsholm, Denmark.

Abstract

Second-generation genome sequencing and alignment of the resulting reads to in silico genomes containing antimicrobial resistance and virulence factor genes were used to screen for undesirable genes in 28 strains which could be used in human nutrition. No virulence factor genes were detected, while several isolates contained antimicrobial resistance genes.

MeSH terms

Anti-Bacterial Agents / pharmacology
Base Sequence
Bifidobacterium / drug effects
Bifidobacterium / genetics
Drug Resistance, Bacterial*
Drug Resistance, Multiple, Bacterial / drug effects
Drug Resistance, Multiple, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics
Genome, Bacterial*
Lactobacillus / drug effects
Lactobacillus / genetics
Lactococcus lactis / drug effects
Lactococcus lactis / genetics
Microbial Sensitivity Tests / methods*
Sequence Alignment
Sequence Analysis, DNA / methods*
Streptococcus / drug effects
Streptococcus / genetics
Virulence Factors / genetics*

Substances

Anti-Bacterial Agents
Virulence Factors