Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua

Trond Løvdal; Maria Befring Hovda; Benny Björkblom; Simon G Møller

doi:10.1016/j.mimet.2011.01.027

Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua

J Microbiol Methods. 2011 May;85(2):164-9. doi: 10.1016/j.mimet.2011.01.027. Epub 2011 Feb 12.

Authors

Trond Løvdal¹, Maria Befring Hovda, Benny Björkblom, Simon G Møller

Affiliation

¹ Nofima Norconserv AS, Stavanger, Norway. trond.lovdal@nofima.no

PMID: 21324348
DOI: 10.1016/j.mimet.2011.01.027

Abstract

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Azides / chemistry
Colony Count, Microbial
Hot Temperature
Listeria / chemistry
Listeria / genetics
Listeria / growth & development*
Listeria / isolation & purification
Microbial Viability*
Polymerase Chain Reaction / methods*
Propidium / analogs & derivatives
Propidium / chemistry
Staining and Labeling / methods*

Substances

Azides
propidium monoazide
Propidium