High-resolution melting curve analysis to establish CYP2C19∗2 single nucleotide polymorphism: Comparison with hydrolysis SNP analysis

Manna Temesvári; József Paulik; László Kóbori; Katalin Monostory

doi:10.1016/j.mcp.2011.01.003

High-resolution melting curve analysis to establish CYP2C19∗2 single nucleotide polymorphism: Comparison with hydrolysis SNP analysis

Mol Cell Probes. 2011 Apr-Jun;25(2-3):130-3. doi: 10.1016/j.mcp.2011.01.003. Epub 2011 Feb 15.

Authors

Manna Temesvári¹, József Paulik, László Kóbori, Katalin Monostory

Affiliation

¹ Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri 59-67, H-1025 Budapest, Hungary. m.temesvari@chemres.hu

PMID: 21315147
DOI: 10.1016/j.mcp.2011.01.003

Abstract

Clinically significant consequences of CYP2C19 polymorphism in drug metabolism require reliable genotyping methods for assaying large numbers of blood samples. Hydrolysis single nucleotide polymorphism analysis and high-resolution melting curve (HRM) analysis successfully genotyped the 114 liver donors for CYP2C19∗2. However, the HRM analysis was found to be more efficient, rapid, user- and cost-friendly method.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Aryl Hydrocarbon Hydroxylases / genetics*
Aryl Hydrocarbon Hydroxylases / metabolism
Cytochrome P-450 CYP2C19
DNA Primers / genetics
Genotype
Humans
Hydrolysis
Liver Transplantation
Microsomes, Liver / metabolism
Nucleic Acid Denaturation*
Polymerase Chain Reaction / methods
Polymorphism, Single Nucleotide*
Reproducibility of Results
Tissue Donors

Substances

DNA Primers
Aryl Hydrocarbon Hydroxylases
CYP2C19 protein, human
Cytochrome P-450 CYP2C19