Point-of-care testing system enabling 30 min detection of influenza genes

Tomoteru Abe; Yuji Segawa; Hidetoshi Watanabe; Tasuku Yotoriyama; Shinichi Kai; Akio Yasuda; Norio Shimizu; Naoko Tojo

doi:10.1039/c0lc00519c

Point-of-care testing system enabling 30 min detection of influenza genes

Lab Chip. 2011 Mar 21;11(6):1166-7. doi: 10.1039/c0lc00519c. Epub 2011 Feb 11.

Authors

Tomoteru Abe¹, Yuji Segawa, Hidetoshi Watanabe, Tasuku Yotoriyama, Shinichi Kai, Akio Yasuda, Norio Shimizu, Naoko Tojo

Affiliation

¹ Sony Corporation, Life Science Laboratory, Advanced Materials Laboratories, Tokyo Medical and Dental University, 1-5-45 Yushima, Tokyo 113-8510, Japan.

PMID: 21311813
DOI: 10.1039/c0lc00519c

Abstract

We developed a portable and easy-to-use nucleic acid amplification test (NAT) system for use in point-of-care testing (POCT). The system shows sensitivity that is sufficiently higher than that of the currently available rapid diagnostic kit and is comparable to that of real-time reverse transcription polymerase chain reaction (RT-PCR) for influenza testing.

MeSH terms

Humans
Influenza A virus / genetics
Influenza A virus / isolation & purification*
Influenza, Human / diagnosis
Nucleic Acid Amplification Techniques / methods*
Point-of-Care Systems*
RNA, Viral / analysis
Reverse Transcriptase Polymerase Chain Reaction

Substances

RNA, Viral