Fast cleanup system combined with a dioxin-responsive element-driven luciferase bioassay for analysis of polychlorinated dibenzo-p-dioxins/furans in sediments and soils

Bull Environ Contam Toxicol. 2011 Mar;86(3):278-82. doi: 10.1007/s00128-011-0205-3. Epub 2011 Feb 5.

Abstract

A green technique was designed for assessing toxic equivalence (TEQ) levels of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in sediments and soil from dioxin-contaminated areas. This technique combines a fast cleanup system with a dioxin-responsive-element (DRE)-driven luciferase bioassay. Samples from sediment (n = 10) and soil (n = 11) were analyzed with the technique; levels of PCDD/Fs ranged from 75.1 to 2,670 ng DRE-driven luciferase activity (DL)-TEQ/kg dry weight (d.w.). Significant correlations were found between DL-TEQs (by the bioassay) and PCDD/F WHO-TEQs (by HRGC/HRMS). DL-TEQs is significantly correlated with WHO-TEQs of 2,3,7,8-TCDD and 1,2,3,7,8-PeCDD using a multiple stepwise linear regression model (adjusted R(2) = 0.962, p < 0.001).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzofurans / analysis*
  • Benzofurans / chemistry
  • Biological Assay / methods
  • Dibenzofurans, Polychlorinated
  • Endocrine Disruptors / analysis
  • Endocrine Disruptors / chemistry
  • Environmental Monitoring / methods*
  • Geologic Sediments / chemistry
  • Green Chemistry Technology / methods
  • Linear Models
  • Luciferases / chemistry*
  • Multivariate Analysis
  • Polychlorinated Dibenzodioxins / analogs & derivatives*
  • Polychlorinated Dibenzodioxins / analysis
  • Polychlorinated Dibenzodioxins / chemistry
  • Soil / chemistry
  • Soil Pollutants / analysis*
  • Soil Pollutants / chemistry

Substances

  • Benzofurans
  • Dibenzofurans, Polychlorinated
  • Endocrine Disruptors
  • Polychlorinated Dibenzodioxins
  • Soil
  • Soil Pollutants
  • Luciferases