Specific biotinylation of IMP dehydrogenase

Bioorg Med Chem Lett. 2011 Mar 1;21(5):1363-5. doi: 10.1016/j.bmcl.2011.01.042. Epub 2011 Jan 14.

Abstract

IMP dehydrogenase (IMPDH) catalyzes a critical step in guanine nucleotide biosynthesis. IMPDH also has biological roles that are distinct from its enzymatic function. We report a biotin-linked reagent that selectively labels IMPDH and is released by dithiothreitol. This reagent will be invaluable in elucidating the moonlighting functions of IMPDH.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Biotinylation
  • Dithiothreitol / chemistry
  • Humans
  • IMP Dehydrogenase / chemistry*
  • Models, Molecular
  • Molecular Structure

Substances

  • IMP Dehydrogenase
  • Dithiothreitol