Abstract
There is increasing evidence that microRNAs (miRNAs) play important roles in cell proliferation, apoptosis and differentiation that accompany inflammatory responses. However, whether microRNAs are associated with DC immuno-inflammatory responses with oxidized low density lipoprotein (oxLDL) stimulation is not yet known. Our study aims to explore the link of miRNAs with lipid-overload and immuno-inflammatory mechanism for atherosclerosis. In DCs transfected with microRNA-29a mimics or inhibitors, we showed that microRNA-29a plays an important role in proinflammatory cytokine secretion and scavenger receptor expression upon oxLDL-treatment. Furthermore, we suggest an additional explanation for the mechanism of microRNA-29a regulation of its functional target, lipoprotein lipase. We conclude that microRNA-29a could regulate pro-inflammatory cytokine secretion and scavenger receptor expression by targeting lipoprotein lipase in oxLDL-stimulated dendritic cells.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Atherosclerosis / drug therapy
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Atherosclerosis / immunology
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CD36 Antigens / genetics
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CD36 Antigens / metabolism
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Cells, Cultured
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Cytokines / metabolism*
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Dendritic Cells / drug effects
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Dendritic Cells / immunology
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Dendritic Cells / metabolism*
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Down-Regulation*
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Gene Expression Regulation*
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Genes, Reporter
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Humans
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Lipoprotein Lipase / genetics
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Lipoprotein Lipase / metabolism*
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Lipoproteins, LDL / metabolism
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MicroRNAs / antagonists & inhibitors
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Molecular Targeted Therapy
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Oxidation-Reduction
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RNA, Messenger / metabolism
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Receptors, Scavenger / metabolism*
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Scavenger Receptors, Class A / genetics
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Scavenger Receptors, Class A / metabolism
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Scavenger Receptors, Class E / genetics
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Scavenger Receptors, Class E / metabolism
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Transfection
Substances
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3' Untranslated Regions
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CD36 Antigens
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Cytokines
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Lipoproteins, LDL
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MIRN29a microRNA, human
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MicroRNAs
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OLR1 protein, human
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RNA, Messenger
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Receptors, Scavenger
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Scavenger Receptors, Class A
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Scavenger Receptors, Class E
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oxidized low density lipoprotein
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LPL protein, human
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Lipoprotein Lipase