An electrochemical approach for detection of DNA methylation and assay of the methyltransferase activity

Chem Commun (Camb). 2011 Mar 14;47(10):2844-6. doi: 10.1039/c0cc05153e. Epub 2011 Jan 21.

Abstract

This work develops an electrochemical approach for rapid detection of the genomic DNA methylation level, assay of methyltransferase activity, and evaluation and screening of the inhibitors of methyltransferase. This method may be a help for the discovery of anticancer drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • CpG Islands
  • DNA / chemistry
  • DNA / genetics*
  • DNA / metabolism
  • DNA Methylation*
  • Electrochemistry / methods*
  • Enzyme Assays / methods*
  • Methyltransferases / metabolism*
  • Models, Molecular
  • Nucleic Acid Conformation
  • Time Factors

Substances

  • DNA
  • Methyltransferases