[Establishment of a human large cell lung cancer cell line L9981-nm23-H1]

Liangan Che; Qinghua Zhou; Yanping Wang; Jun Chen; Lunxu Liu; Yang Qin; Zhilin Sun; Xiaohe Chen; Wen Zhu

doi:10.3779/j.issn.1009-3419.2004.03.01

[Establishment of a human large cell lung cancer cell line L9981-nm23-H1]

Zhongguo Fei Ai Za Zhi. 2004 Jun 20;7(3):187-90. doi: 10.3779/j.issn.1009-3419.2004.03.01.

[Article in Chinese]

Authors

Liangan Che¹, Qinghua Zhou, Yanping Wang, Jun Chen, Lunxu Liu, Yang Qin, Zhilin Sun, Xiaohe Chen, Wen Zhu

Affiliation

¹ The Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.

PMID: 21232214
DOI: 10.3779/j.issn.1009-3419.2004.03.01

Abstract

Background: To establish a human large cell lung cancer cell line L9981-nm23-H1 transfected with wild type nm23-H1 gene.

Methods: pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and L9981-nm23-H1 was established by infected virus of nm23-H1 gene. The DNA and protein expression of nm23-H1 were detected in the transgene large cell lung cancer cell line by PCR and Western blot.

Results: pLXSN-nm23-H1-EGFP was constructed successfully, and the nm23-H1 cDNA was inducted to L9981 cell. The protein of nm23-H1 could be detected in L9981-nm23-H1 cell.

Conclusions: Protein of nm23-H1 is stably, continuously and high efficiently expressed in L9981-nm23-H1 cell.

Publication types

English Abstract