Abstract
Objective:
To construct the cell model of epithelium to mesenchymal transition of proximal tubule cells induced by high glucose and to determine the expression of Smad anchor for receptor activation (SARA).
Methods:
Protein expression of vimentin, Zona occludens-1(ZO-1), and SARA was determined by Western blot, and their mRNA expressions were detected by Real-time PCR.
Results:
After stimulation by 30 mmol/L D-glucose, the protein and mRNA expression levels of vimentin in HK-2 cells increased in a time-dependent manner while the expression of ZO-1 was reduced significantly, especially at 48 h. Meanwhile, SARA was also decreased in a time-dependent manner.
Conclusion:
High glucose can induce renal epithelium to mesenchymal transition, and SARA may be involved in this process as a protector.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Differentiation
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Cells, Cultured
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Epithelial Cells / cytology*
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Glucose / pharmacology*
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Kidney Tubules, Proximal / cytology*
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Kidney Tubules, Proximal / metabolism
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Membrane Proteins / metabolism
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Mesoderm / cytology*
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Phosphoproteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Serine Endopeptidases / genetics
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Serine Endopeptidases / metabolism*
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Signal Transduction
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Transforming Growth Factor beta1 / pharmacology
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Vimentin / metabolism
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Zonula Occludens-1 Protein
Substances
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Phosphoproteins
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RNA, Messenger
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TJP1 protein, human
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Transforming Growth Factor beta1
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Vimentin
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Zonula Occludens-1 Protein
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ZFYVE16 protein, human
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Serine Endopeptidases
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Glucose