Abstract
We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agrobacterium tumefaciens / enzymology*
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Agrobacterium tumefaciens / genetics
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Agrobacterium tumefaciens / metabolism
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Alkanes / metabolism
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Esters / metabolism
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Hydrolases / genetics
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Hydrolases / isolation & purification*
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Hydrolases / metabolism*
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Molecular Sequence Data
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Plants / microbiology
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Protein Multimerization
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Sequence Analysis, DNA
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Stereoisomerism
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Substrate Specificity
Substances
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Alkanes
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DNA, Bacterial
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Esters
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Hydrolases
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haloalkane dehalogenase