Photoelectron transport ability of chloroplast thylakoid membranes treated with NO donor SNP: changes in flash oxygen evolution and chlorophyll fluorescence

Radka Vladkova; Anelia G Dobrikova; Ranjeet Singh; Amarendra N Misra; Emilia Apostolova

doi:10.1016/j.niox.2010.12.003

Photoelectron transport ability of chloroplast thylakoid membranes treated with NO donor SNP: changes in flash oxygen evolution and chlorophyll fluorescence

Nitric Oxide. 2011 Mar 15;24(2):84-90. doi: 10.1016/j.niox.2010.12.003. Epub 2010 Dec 15.

Authors

Radka Vladkova¹, Anelia G Dobrikova, Ranjeet Singh, Amarendra N Misra, Emilia Apostolova

Affiliation

¹ Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Science, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.

PMID: 21167290
DOI: 10.1016/j.niox.2010.12.003

Abstract

The nitric oxide (NO) donor sodium nitroprusside (SNP) is frequently used in plant science in vivo. The present in vitro study reveals its effects on the photosynthetic oxygen evolution and the chlorophyll fluorescence directly on isolated pea thylakoid membranes. It was found that even at very low amounts of SNP (chlorophyll/SNP molar ratio∼67:1), the SNP-donated NO stimulates with more than 50% the overall photosystem II electron transport rate and diminishes the evolution of molecular oxygen. It was also found that the target site for SNP-donated NO is the donor side of photosystem II. Compared with other NO-donors used in plant science, SNP seems to be the only one exhibiting stimulation of electron transport through photosystem II.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Chlorophyll / chemistry*
Electrons*
Fluorescence
Light*
Nitric Oxide Donors / pharmacology*
Nitroprusside / pharmacology*
Oxygen / metabolism*
Pisum sativum / physiology*
Thylakoids / drug effects*
Thylakoids / metabolism*

Substances

Nitric Oxide Donors
Chlorophyll
Nitroprusside
Oxygen