Denaturing gradient gel electrophoresis analysis with different primers of subgingival bacterial communities under mechanical debridement

Dali Liu; Yanbin Zhou; Chaolun Li; Yunpeng Li; Yuntao Jiang; Zhengwei Huang; Jingping Liang; Rong Shu

doi:10.1111/j.1348-0421.2010.00261.x

Denaturing gradient gel electrophoresis analysis with different primers of subgingival bacterial communities under mechanical debridement

Microbiol Immunol. 2010 Nov;54(11):702-6. doi: 10.1111/j.1348-0421.2010.00261.x.

Authors

Dali Liu¹, Yanbin Zhou, Chaolun Li, Yunpeng Li, Yuntao Jiang, Zhengwei Huang, Jingping Liang, Rong Shu

Affiliation

¹ Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.

PMID: 21155359
DOI: 10.1111/j.1348-0421.2010.00261.x

Abstract

DGGE of 16S rDNA is one of the most frequently used methods to study microbial communities. In this study, the DGGE profiles of different 16S rDNA regions of the periodontal pathogens Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella nigrescens were investigated. The results suggested that V3-V5 and V6-V8 fragments may be suitable for community analysis of subgingival bacteria. Further analysis of subgingival samples with V3-V5 and V6-V8 regions as target fragments suggested that, in chronic periodontitis, re-colonization by periodontal bacteria with a population very similar to the baseline may occur by 6 weeks after mechanical debridement.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Bacteria / isolation & purification*
Chronic Disease
DNA Primers
DNA, Bacterial / analysis
DNA, Ribosomal / analysis
Denaturing Gradient Gel Electrophoresis / methods*
Female
Gingiva / microbiology*
Humans
Male
Middle Aged
Periodontal Debridement*
Periodontitis / microbiology*
RNA, Ribosomal, 16S / genetics

Substances

DNA Primers
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S