Penzigomyces catalonicus sp. nov., collected on dead branches of an unknown tree species in Spain, is described and illustrated. This fungus is characterized by short, dark brown, percurrent conidiophores, usually doliiform conidiogenous cells and ellipsoidal or slightly obclavate, (2-)3-4(-6) euseptate, thin-walled conidia. The new taxon is compared with species from other morphologically similar genera, and a key to the known species of Penzigomyces is provided.