Surface microstructure and cell compatibility of calcium silicate and calcium phosphate composite coatings on Mg-Zn-Mn-Ca alloys for biomedical application

Colloids Surf B Biointerfaces. 2011 Mar;83(1):96-102. doi: 10.1016/j.colsurfb.2010.11.003. Epub 2010 Nov 9.

Abstract

A calcium silicate and calcium phosphate (CaSiO(3)/CaHPO(4)·2H(2)O) composite coating was applied by a chemical reaction to Mg-Zn-Mn-Ca alloy to improve its biocompatiblity. The surface microstructure was observed by scanning electronic microscopy (SEM) and the surface bioactivity was assessed by a cell interaction experiment. SEM observation showed that a microporous layer was formed on the surface of Mg-Zn-Mn-Ca alloy. It was shown by XRD that the reaction layer was mainly composed of CaHPO(4)·2H(2)O and a small amount of CaSiO(3). In vitro cell experiments indicated that osteoblasts showed good adhesion, high growth rates and proliferation characteristics on the coated Mg-Zn-Mn-Ca alloy, indicating that the surface cytocompatibility of Mg-Zn-Mn-Ca alloy was significantly improved by the calcium phosphate coating.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys / pharmacology*
  • Animals
  • Calcium Compounds / chemistry
  • Calcium Compounds / pharmacology*
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / pharmacology*
  • Cell Cycle / drug effects
  • Cell Proliferation / drug effects
  • Cell Shape / drug effects
  • Cells, Cultured
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / pharmacology*
  • Electrochemical Techniques
  • Hydrogen-Ion Concentration / drug effects
  • Materials Testing / methods*
  • Metals / chemistry*
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects
  • Phase Transition / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Silicates / chemistry
  • Silicates / pharmacology*
  • Surface Properties / drug effects
  • Time Factors
  • X-Ray Diffraction

Substances

  • Alloys
  • Calcium Compounds
  • Calcium Phosphates
  • Coated Materials, Biocompatible
  • Metals
  • Silicates
  • calcium phosphate
  • calcium silicate